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anti kap1  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc anti kap1
    Anti Kap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+kap1/TIF1beta+Rabbit+mAb/pm41690451-225-45-49
    Average 94 stars, based on 50 article reviews
    anti kap1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Radio Immunoprecipitation:

    Article Title: CtIP is essential for early B cell proliferation and development in mice
    Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40, and fresh proteinase inhibitor cocktail) following standard Western blotting protocols and blotted with antibodies specific for Flag (M2, 1:5,000, F3165; Sigma-Aldrich), CtIP (1:1,000; ), anti-pho-KAP1 (1:1,000, A300-767A; Bethyl Laboratories), anti-KAP1 (1:1,000, 4124S; Cell Signaling), anti-CHK1 (1:1,000, 2348S; Cell Signaling), anti-pho-CHK1(S345) (1:1,000, 2348S; Cell Signaling), anti-RPA32 (1:10,000, A300-244A; Bethyl Laboratories), and anti-pho-RPA(T21) (1:10,000, ab19109394; Abcam). .. HRP-conjugated anti-rabbit and mouse secondary antibodies (GE Healthcare) were used, and an ECL Western blotting detection system (GE Healthcare) was used for detection.

    Western Blot:

    Article Title: CtIP is essential for early B cell proliferation and development in mice
    Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40, and fresh proteinase inhibitor cocktail) following standard Western blotting protocols and blotted with antibodies specific for Flag (M2, 1:5,000, F3165; Sigma-Aldrich), CtIP (1:1,000; ), anti-pho-KAP1 (1:1,000, A300-767A; Bethyl Laboratories), anti-KAP1 (1:1,000, 4124S; Cell Signaling), anti-CHK1 (1:1,000, 2348S; Cell Signaling), anti-pho-CHK1(S345) (1:1,000, 2348S; Cell Signaling), anti-RPA32 (1:10,000, A300-244A; Bethyl Laboratories), and anti-pho-RPA(T21) (1:10,000, ab19109394; Abcam). .. HRP-conjugated anti-rabbit and mouse secondary antibodies (GE Healthcare) were used, and an ECL Western blotting detection system (GE Healthcare) was used for detection.

    Article Title: M1C Mediates LINE-1 Transcription in PARP Inhibitor-Treated Prostate Cancer Cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Immunoprecipitation:

    Article Title: Role of Tet proteins in enhancer activity and telomere elongation
    Article Snippet: .. Immunoprecipitation was performed with anti-Kap1 (Cell Signaling, 4123), anti-Ring1b (Cell Signaling, 5694), anti-Ezh2 (Cell Signaling, 5246), or rabbit IgG (Santa Cruz Biotechnology, SC-2027). ..

    Incubation:

    Article Title: Interferon regulatory factor 8 regulates caspase-1 expression to facilitate Epstein-Barr virus reactivation in response to B cell receptor stimulation and chemical induction
    Article Snippet: .. Membranes were then incubated in the following primary antibodies: mouse anti-ZTA (Argene, Cat # 11–007, 1:5,000), mouse anti-RTA (Argene, 1:1,000), mouse anti-BGLF4 antibody (1:1,000) [ ], anti-β-actin (Sigma, Cat # A5441, 1:5,000), anti-IRF8 (CST, Cat #5628, 1:1,000), anti-PARP (CST, Cat #9532, 1:1,000), anti-Cleaved PARP (CST, Cat #5625, 1:1,000), anti-Cleaved Caspase Substrates (CST, Cat #8698, 1:1,000), anti-Caspase-1 (CST, Cat #3866, 1:1,000), anti-Caspase-2 (CST, Cat #2224, 1:1,000), anti-Caspase-3 (Santa Cruz, Cat #sc-7148, 1:1,000), anti-Cleaved Caspase-3 (CST, Cat #9664, 1:1,000), anti-Caspase-7 (CST, Cat #12827, 1:1,000), anti-Cleaved Caspase-7 (CST, Cat #8438, 1:1,000), anti-Caspase-8 (CST, Cat #9746, 1:1,000), anti-Cleaved Caspase-8 (CST, Cat #9496, 1:1,000), anti-Caspase-9 (CST, Cat #9508, 1:1,000), anti-Bcl-2 (Bethyl, Cat #A303-675A, 1:1,000), anti-KAP1 (CST, Cat #4123, 1:1,000), anti-PAX5 (CST, Cat #8970, 1:1,000), anti-DNMT3A (Bethyl, Cat #A304-278A, 1:1,000), anti-STAT3 (CST, Cat #9139, 1:1,000), and anti-HA (CST, Cat #14031S, 1:1,000). .. The secondary antibodies used were horseradish peroxidase (HRP)-labeled goat anti-mouse antibody (Fisher Scientific, 1:5,000) and HRP-labeled anti-rabbit antibody (Fisher scientific, 1:5,000).



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